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| 產(chǎn)地 | 北京 |
| 品牌 | solarbio |
| 貨號(hào) | C0060 |
| 用途 | DAPI(4’,6-二脒基-2-苯基吲哚二鹽酸鹽)是一種能夠與DNA中大部分A，T堿基相互結(jié)合的熒光染料﹐常用于熒光顯微鏡觀測(cè)。 |
| 包裝規(guī)格 | 1ml/1ml*10 |
| CAS編號(hào) | 28718-90-3 |
| 純度 | ≥90% (HPLC和 TLC)% |
| 是否進(jìn)口 | 否 |
產(chǎn)品簡(jiǎn)介
1.中文名：DAPI溶液（1mg/ml）
2.英文名：DAPI
3.別 名：4',6-二脒基-2-苯基吲哚
4.貨 號(hào)：C0060
5.品 牌：solarbio
6.儲(chǔ)存條件：-20℃，有效期至少 2 年
7.用 途 : 產(chǎn)品說明：
DAPI(4’,6-二脒基-2-苯基吲哚*)是一種能夠與 DNA 中大部分 A，T 堿基相互結(jié)合的熒光染料﹐常用于熒光顯微鏡觀測(cè)。因?yàn)?nbsp;DAPI 可以透過完整的細(xì)胞膜﹐它可以用于活細(xì)胞和固定細(xì)胞的染色。當(dāng) DAPI 與雙鏈 DNA 結(jié)合時(shí)，大吸收波長(zhǎng)為 358nm，大發(fā)射波長(zhǎng)為 461nm。DAPI 的發(fā)射光為藍(lán)色，且 DAPI 和綠色熒光蛋白 GFP 或 Texas Red 染劑(紅色熒光染劑)的發(fā)射波長(zhǎng)僅有少部分重疊，可以利用這項(xiàng)特性在單一的樣品上進(jìn)行多重?zé)晒馊旧?nbsp;本產(chǎn)品為 DAPI 水溶液，純度≥90%，濃度為 1mg/ml。使用時(shí)根據(jù)實(shí)驗(yàn)不同直接將本產(chǎn)品用相應(yīng)溶液稀釋到工作濃度。
使用方法： （僅供參考）
z 對(duì)于培養(yǎng)細(xì)胞
1， 取適量 DAPI 水溶液加到 PBS 中，制備成 5-15 μg/ml 的 DAPI 溶液。
2， 將 1/10 培養(yǎng)基體積的 DAPI 溶液加入到細(xì)胞培養(yǎng)基中。
3， 在 37℃培養(yǎng)細(xì)胞 10-20 分鐘。
4， 用 PBS 或合適的緩沖液洗細(xì)胞兩次。
5， 置于熒光顯微鏡下觀察，激發(fā)波長(zhǎng) 360-400nm。
z 對(duì)于組織切片 制好的玻片上滴加幾滴稀釋的 DAPI 染液，染色 10 分鐘，流水沖去染液，濾紙吸除多余水分，加一滴熒光封片液，置于熒光顯微鏡下觀察。
注意事項(xiàng)：
DAPI 被普遍認(rèn)為具有致癌性，操作時(shí)應(yīng)戴手套，并避免交叉污染。
本產(chǎn)品需避光，并盡量避免反復(fù)凍融。
相關(guān)產(chǎn)品：
C0080 碘化丙錠 PI溶液（1mg/ml）
CA1020 ANNEXIN V-FITC/PI凋亡檢測(cè)試劑盒
12100 DMEM(H)培養(yǎng)基
S9000 特級(jí)胎牛血清
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