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| 產(chǎn)地 | 北京 |
| 品牌 | solarbio |
| 貨號 | C0065 |
| 用途 | 與DNA中大部分A，T堿基相互結(jié)合的熒光染料 |
| 包裝規(guī)格 | 10ml/50ml |
| CAS編號 | 28718-90-3 |
| 純度 | ≥90% (HPLC和 TLC)% |
| 是否進口 | 否 |
產(chǎn)品說明：
DAPI(4’,6- 二脒基 -2- 苯基吲哚* ) 是一種能夠與 DNA 中大部分 A ， T 堿基相互結(jié)合的熒光染料﹐常用于熒光顯微鏡觀測。因為 DAPI 可以透過完整的細胞膜﹐它可以用于活細胞和固定細胞的染色。當 DAPI 與雙鏈 DNA 結(jié)合時，大吸收波長為 358nm ，大發(fā)射波長為 461nm 。 DAPI 的發(fā)射光為藍色，且 DAPI 和綠色熒光蛋白 GFP 或 Texas Red 染劑 ( 紅色熒光染劑 ) 的發(fā)射波長僅有少部分重疊，可以利用這項特性在單一的樣品上進行多重熒光染色。
Solarbio生產(chǎn)的 DAPI染液分為即用型與濃縮液 （ 濃度為 1mg/ml） 。即用型工作液無需稀釋，可以直接用于固定細胞或組織的細胞核染色。濃縮液使用時根據(jù)實驗不同直接將本產(chǎn)品用相應(yīng)溶液稀釋到工作濃度。
注意事項：
DAPI 被普遍認為具有致癌性，操作時應(yīng)戴手套，并避免交叉污染。
本產(chǎn)品需避光，并盡量避免反復(fù)凍融。
熒光染料都存在淬滅問題，建議染色后盡量當天完成檢測。
為減緩熒光淬滅可以使用 抗熒光衰減封片劑 ，貨號為 S2100 。
相關(guān)產(chǎn)品：
12100 DMEM(H)
31800 RPMI Medium 1640
24800 通用細胞凍存液
H1025 Hanks，含鈣鎂，不含酚紅（HBSS）
P1020 1×PBS，PH7.2-7.4，0.01M，液體
T1300 胰蛋白酶－EDTA消化液(0.25%)不含酚紅
P1400 青鏈霉素混合液(100×)
M1020 MTT細胞增殖及細胞毒性檢測試劑盒
G1080 Mayer'蘇木素染液(免疫組化) 10ml/100ml/500ml
G1100 伊紅染色液 100ml/500ml
G1120 HE染色液(試劑盒) 3×10ml/3×100ml
G1130 莢膜染色液（試劑盒） 2*50/2*100/2*250ml
G1132 芽孢染色液（試劑盒） 2*50/2*100/2*250ml
G1134 鞭毛染色液（試劑盒） 100ml
G1140 Cole氏蘇木素染色液(常規(guī)染色) 100ml/500ml
G1160 石炭酸復(fù)紅染液(苯酚品紅染液) 100ml/500ml
G1165 改良型石炭酸復(fù)紅染液(苯酚品紅染液) 100ml/500ml
G1170 抗酸染色液（試劑盒） 3*50/3*100/3*250ml
G1180 0.1%呂氏堿性美藍染色液 100ml/500ml
G1185 0.4%呂氏堿性美藍染色液 100ml/500ml
G1210 溴甲酚綠-甲基紅指示劑溶液 100ml/500ml
G1220 甲苯胺藍溶液 100ml/500ml
G1260 飽和油紅O染色液 100ml/500ml
G1270 亮綠SF染色液（1%） 100ml/500ml
G1310 中性紅染色液(0.33% 過濾除菌，活細胞染色) 100ml/500ml
G1315 中性紅染色液(0.5% 細胞或組織切片染色) 100ml/500ml
G1316 中性紅染色液(0.1% 常規(guī)染色) 100ml/500ml
G3004 0.4% TTC染色液 100ml/500ml
G8140 GoldView I型核酸染色劑(10000×) 1ml
G8142 GoldView II型核酸染色劑(5000×) 0.5ml
H1020 Hanks，含鈣鎂，含酚紅（HBSS） 500ml
H1025 Hanks，含鈣鎂，不含酚紅（HBSS） 500ml
H1040 D-Hanks，不含鈣鎂，含酚紅（HBSS） 500ml
H1045 D-Hanks，不含鈣鎂，不含酚紅（HBSS） 500ml
H1060 鮭魚精DNA 10mg/ml 1ml
H1080 2×HEPES(PH7.2-7.4，無菌) 100ml
H1090 1M Hepes溶液(Free Acid，無菌) 100ml/500ml
H1095 1M Hepes溶液(PH7.2-7.4，無菌) 100ml/500ml
H1130 7.5% BSA溶液（細胞培養(yǎng)級） 100/200/500ml
I1020 IPTG 溶液 (50mg/ml) 5ml
K1030 Kanamycin (100mg/ml)卡那霉素 5ML/10ml
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