|
| 產(chǎn)地 | 中國(guó) |
| 品牌 | solarbio |
| 貨號(hào) | M1025 |
| 用途 | 現(xiàn)詢客服 |
| 英文名稱 | MTT |
| 包裝規(guī)格 | 5ml |
| 純度 | 現(xiàn)詢客服% |
| CAS編號(hào) | |
| 保質(zhì)期 | 2年 |
| 是否進(jìn)口 | 否 |
產(chǎn)品簡(jiǎn)介
1.中文名：MTT溶液（0.5%）
2.英文名：MTT
3.分 類(lèi)：生化試劑
4.保存條件：RT
5.品 牌：solarbio
MTT溶液（0.5%）的詳細(xì)描述
MTT溶液（0.5%）僅用于科研試驗(yàn)。
MTT溶液（0.5） 染色液
MTT是一種檢測(cè)細(xì)胞存活和生長(zhǎng)的方法。MTT實(shí)驗(yàn)原理為活細(xì)胞線粒體中的琥珀酸脫氫酶能使外源性MTT還原為水不溶性的藍(lán)紫色結(jié)晶甲瓚（Formazan）并沉積在細(xì)胞中，而死細(xì)胞無(wú)此功能。二甲基亞砜（DMSO）能溶解細(xì)胞中的甲瓚，用酶聯(lián)免疫檢測(cè)儀在490nm波長(zhǎng)處測(cè)定其光吸收值，可間接反映活細(xì)胞數(shù)量。
在一定細(xì)胞數(shù)范圍內(nèi)，MTT結(jié)晶形成的量與細(xì)胞數(shù)成正比。根據(jù)測(cè)得的吸光度值(OD值)，來(lái)判斷活細(xì)胞數(shù)量，OD值越大，細(xì)胞活性越強(qiáng)(如果是測(cè)藥物毒性，則表示藥物毒性越小)。該方法已廣泛用于一些生物活性因子的活性檢測(cè)、大規(guī)模的抗腫瘤藥物篩選、細(xì)胞毒性試驗(yàn)以及腫瘤放射敏感性測(cè)定等。它的特點(diǎn)是靈敏度高、經(jīng)濟(jì)。
MTT通常用于.細(xì)胞增殖及細(xì)胞活性測(cè)定以及藥物(也包括其他處理方式如放射線照射)對(duì)體外培養(yǎng)的細(xì)胞毒性的測(cè)定。
缺點(diǎn)：由于MTT經(jīng)還原所產(chǎn)生的甲瓚產(chǎn)物不溶于水，需被溶解后才能檢測(cè)。這不僅使工作量增加，也會(huì)對(duì)實(shí)驗(yàn)結(jié)果的準(zhǔn)確性產(chǎn)生影響，而且溶解甲的有機(jī)溶劑對(duì)實(shí)驗(yàn)者也有損害。
注意事項(xiàng)：
1、在配制和保存的過(guò)程中，容器用鋁箔紙包住。
2、配成的MTT需要無(wú)菌，MTT對(duì)菌很敏感
3、MTT一般現(xiàn)用現(xiàn)配，過(guò)濾后4度避光保存兩周內(nèi)(個(gè)人曾做過(guò)4度避光保存4周的MTT溶液，效果仍然不錯(cuò))有效，或配制成5mg/ml保存在-20度長(zhǎng)期保存，避免反復(fù)凍融，小劑量分裝，用避光袋或是黑紙、錫箔紙包住避光以免分解。當(dāng)MTT變?yōu)榛揖G色時(shí)就*不能再用。
4、MTT有致癌性，用的時(shí)候小心,有條件帶那種透明的簿膜手套.
通常，此法中的MTT濃度為5mg/ml。因此，可以稱取MTT 0.5克，溶于100 ml的磷酸緩沖液（PBS）或無(wú)酚紅的培養(yǎng)基中，用0.22μm濾膜過(guò)濾以除去溶液里的細(xì)菌，放4℃ 避光保存即可。在配制和保存的過(guò)程中，容器用鋁箔紙包住。
MTT溶液（0.5） 染色液
參考文獻(xiàn)：
《Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway》 作者:Panpan Zhang,Lingyue Hua,Huan Hou, Xingyue Du,Zhiqiang He, Menghan Liu, Xiaojuan Hu, Nianlong Yan 期刊:International Journal of Molecular Medicine 影響因子:2.928 PMID:30272329
《MicroRNA-145 inhibits proliferation and promotes apoptosis of HepG2 cells by targeting ROCK1 through the ROCK1/NF-κB signaling pathway.》 作者:R.-K. Wang, X.-M. Shao, J.-P. Yang, H.-L. Yan, Y. Shao 期刊:European Review for Medical and Pharmacological Sciences 影響因子:2.721 PMID:31002128
《Identification of Anticancer Compounds in Gallnuts Through PCA-constructed Secondary Metabolite Map》 作者:Jiayu Gao, Xiao Yang, Jiangxia Hu and Weiping Yin 期刊:International Journal of Pharmacology 影響因子:0.754 PMID:
《Epigenetically upregulated oncoprotein PLCE1 drives esophageal carcinoma angiogenesis and proliferation via activating the PI-PLCε-NF-κB signaling pathway and VEGF-C/ Bcl-2 expression》 作者:Yunzhao Chen, Dandan Wang, Hao Peng, Xi Chen, Xueping Han, Jie Yu, Wenjie Wang, Lirong Liang, Zheng Liu, Yi Zheng, Jianming Hu, Lan Yang, Jun Li, Hong Zhou, Xiaobin Cui and Feng Li 期刊:Molecular Cancer 影響因子:10.679 PMID:30609930
《Apoptosis of human hepatocellular carcinoma cells SMMC-7721 induced by C-3 methylidene thiazolidinedione acetic acid》 作者:Hong-Xia Liang, Yi-Hua Yu, Xiao-Hua Li, Nai-Fu Tang, Guo-Qiang Hu, Bin Liu 期刊:Int J Clin Exp Med 影響因子:0.181 PMID:
《Diterpene pekinenal from euphorbia pekinensis radix induced IEC-6 cells apoptosis mediated by mitochondria and death receptors》 作者:FangYaoDongjingJiangFangfangChengWeifengYaoPeidongChenShuchenGuoYudanCaoLiZhang 期刊:Toxicology in Vitro 影響因子:3.105 PMID:30710624
《1-Hydroxy-3,7,8-Trimethoxyxanthone Suppresses the Malignant Proliferation of Human Bone Marrow Mesenchymal Stem Cells in Colon Cancer Microenvironment》 作者:Hui-qiao Zhao, Nian-hua Lu, Xu-dong Zhang, Na Liu and Ming Jing 期刊:International Journal of Pharmacology 影響因子:0.765 PMID:
《Carbon dots serve as an effective probe for the quantitative determination and for intracellular imaging of mercury(II)》 作者:Fanyong Yan,Depeng Kong,Yunmei Luo,Qianghua Ye,Juanjuan He,Xingfei Guo,Li Chen 期刊:Microchimica Acta 影響因子:5.705 PMID:
《LncRNA SNHG15 acts as a ceRNA to regulate YAP1-Hippo signaling pathway by sponging miR-200a-3p in papillary thyroid carcinoma》 作者:DM Wu, S Wang, X Wen, XR Han, YJ Wang 期刊:Cell death & Disease 影響因子:8.371 PMID:30237435
《Effects of tumor-specific antigen induced by lentinan on murine H22 hepatocellular carcinoma immunoprophylaxis》 作者:Y. WANG1, X. HAN， Y.-D. LI， S.-Y. ZHAO, D.-J. ZHANG,Z.-H. ZHAO, Y.-B. WANG 期刊:European Review for Medical and Pharmacological Sciences 影響因子:1.093 PMID:26698247
《Effect of cell culture models on the evaluation of anticancer activity and mechanism analysis of the potential bioactive compound, iturin A, produced by Bacillus subtilis》 作者:H Zhao, X Zhao, S Lei, Y Zhang, D Shao, C Jiang 期刊:Food & function 影響因子:3.289 PMID:30778489
《IL-17A/IL-17RA promotes invasion and activates MMP-2 and MMP-9 expression via p38 MAPK signaling pathway in non-small cell lung cancer》 作者:Zhenhua Wu,Dan He,Shuyuan Zhao,Hongjiang Wang 期刊:Molecular and cellular biochemistry 影響因子:3.062 PMID:30564960
《SIRT1 expression regulates the transformation of resistant esophageal cancer cells via the epithelial-mesenchymal transition》 作者:Tiantian Qin,Weihua Liu,Junfeng Huo,Leilei Li,Xueyan Zhang,Xiaoli Shi,Jinlei Zhou,Cong Wang 期刊:Biomedicine & Pharmacotherapy 影響因子:2.068 PMID:29656187
《Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells》 作者:Shengnan Guo,Yuanyuan Wang, Yu Gao,Yinxu Zhang, Mingzi Chen, Minghao Xu, Lu Hu, Yu Jing, Fangyu Jing, Chen Li, Qingjun Wang, and Zhitu Zhu 期刊:International Medical Journal of Experimental 影響因子:1.42 PMID:27774979
《Transport of curcumin derivatives in Caco-2 cell monolayers》 作者:Zhen Zeng,ZheL.Shen,Shuo Zhai,JiaL.Xu,Hui Liang,Qin Shen,QingY.Lia 期刊:European Journal of Pharmaceutics and Biopharmaceutics 影響因子:4.44 PMID:28396278
《Excitation-independent yellow-fluorescent nitrogen-doped carbon nanodots for biological imaging and paper-based sensing》 作者:Lihong Shi,Lin Li,Xiaofeng Li,Guomei Zhang,Yan Zhang,Chuan Dong,Shaomin Shuang, 期刊:Sensors and Actuators B: Chemical 影響因子:5.667 PMID:
《Nanodiamond-conjugated transferrin as chemotherapeutic drug delivery》 作者:Zhiqin Wang,Zhimei Tian,Yu Dong,Lin Li,Lu Tian,Yingqi Li,Binsheng Yang 期刊:Diamond and Related Materials 影響因子:2.232 PMID:24998271
《Toxicity of imidazoles ionic liquid [C16mim]Cl to Hela cells》 作者:Xiaohua Xi,Ruyan Wan,Peijin Wang,Weiran Huo,Hui Dong,Qiyan Du 期刊:Ecotoxicology and Environmental Safety 影響因子:3.974 PMID:30015186
《線粒體靶向抗氧化劑MitoQ 的抗腫瘤效應(yīng)》 作者:王華偉， 王華南， 康肖夢(mèng)， 張宇睿， 楊福軍… 期刊:輻射研究與輻射工藝 影響因子:0.471 PMID:
《RNAi構(gòu)建parp-1基因低表達(dá)的石英惡性轉(zhuǎn)化細(xì)胞模型》 作者:郭偉， 宋珊珊， 田琳， 高艾， 牛丕業(yè)， 左昕 期刊:首都醫(yī)科大學(xué)學(xué)報(bào) 影響因子:1.511 PMID:
《具有相同陽(yáng)離子鏈段的可生物降解的共聚物及其在siRNA遞送中的性能》 作者:若古奇ab施劉一b杰陳一海嘯一bLesan Yan ab黃玉斌黃一 Xiabin 靖一 期刊:控制釋放 期刊 影響因子: PMID:
《Simultaneous determination of saikosaponins in Bupleurum Radix from different locations by high performance liquid chromatography- charged aerosol detection (HPLC-CAD) method and its immunomodulation effects on mouse splenocytes》 作者:Lina Wang, Bin Chen, Wei Wang, Nan Xu and Tianzhu Jia* 期刊:academic Journals 影響因子: PMID:
《Ras-association domain family 1 (RASSF1A) gene regulates progression, migration and invasion of bladder cancer》 作者:Yuhai Bao,Xuefeng Liu,Yang Liu,Si Wang,Bin Wu 期刊:Surgical Oncology 影響因子:2.136 PMID:
《Protective Effect of Ginsenoside Rg1 on Oxidative Damage Induced by Hydrogen Peroxide in Chicken Splenic Lymphocytes》 作者:Shicheng Bi, Xiaodan Ma, Yuemin Wang, Xiaoqing Chi, Yong Zhang, Wei Xu, and Songhua Hu 期刊:Oxidative Medicine and Cellular Longevity 影響因子:4.868 PMID:31178974
《The role and mechanism of transforming growth factor beta 3 in human myocardial infarction‐induced myocardial fibrosis》 作者:Ke Xue , Jun Zhang , Cong Li , Jing Li , Cong Wang, Qingqing Zhang, Xianlu Chen , Xiaotang Yu , Lei Sun ,Xiao Yu 期刊:Journal of Cellular and Molecular Medicine 影響因子:4.658 PMID:30983140
《TRPC5?induced autophagy promotes the TMZ?resistance of glioma cells via the CAMMKβ/AMPKα/mTOR pathway》 作者:Yan Zou, Mu Chen, Shuai Zhang ,Zeng'li Miao, Jing Wang ,Xiao'jie Lu ,Xu'dong Zhao 期刊:Oncology Reports 影響因子:3.041 PMID:30942446
《Curcumin analogues attenuate Aβ25-35-induced oxidative stress in PC12 cells via Keap1/Nrf2/HO-1 signaling pathways》 作者:Jialin Xu，Leilei Zhou，QiWeng，Linxia Xiao，Qingyong Li 期刊:Chemico-Biological Interactions 影響因子:3.407 PMID:30946834
《3D carbon nanosphere and gold nanoparticle-based voltammetric cytosensor for cell line A549 and for early diagnosis of non-small cell lung cancer cells》 作者:Huan Zhang，Hong Ke，Yinfang Wang，Pengwei Li，Chusen Huang，Nengqin Jia 期刊:Microchimica Acta 影響因子:5.479 PMID:30569315
《Autocrine secretions enhance radioresistance in an exosome?independent manner in NSCLC cells》 作者:Shuang Wang， Piaoyang Gao ，Na Li ，Ping Chen ，Jinhan Wang， Ningning He，Kaihua Ji， Liqing Du ，Qiang Liu 期刊:International Journal of Oncology 影響因子:3.571 PMID:30387839
《Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa》 作者:Hai-yu Ji,Pei Chen,Juan Yu,Ying-ying Feng,An-jun Liu 期刊:Applied Biochemistry and Biotechnology 影響因子:2.14 PMID:30536035
《Cucurbit[7]uril enhances photosensitization of porphyrins in Neuroblastoma cells》 作者:Xiaoyu Li,Bo Xiao,Yan Guo,Yan Xiao,Song Xiao 期刊:Photodiagnosis and Photodynamic Therapy 影響因子:2.589 PMID:30648639
《Scutellarin protects human retinal pigment epithelial cells against hydrogen peroxide (H2O2)-induced oxidative damage》 作者:Xin Hu, Xiaofang Wu, Bo Zhao and Yongyi Wang 期刊:Cell & Bioscience 影響因子:3.355 PMID:30680088
《Andrographolide prevents human nucleus pulposus cells against degeneration by inhibiting the NF‐κB pathway》 作者:Jianwei Liu , Tongmeng Jiang, Mingwei He , Depeng Fang, Chong Shen, Yiguan Le , Maolin He , Jinmin Zhao, Li Zheng 期刊:Journal of cellular physiology 影響因子:4.522 PMID:30370694
《Honokiol inhibits breast cancer cell metastasis by blocking EMT through modulation of Snail/Slug protein translation》 作者:Wen-die Wang, Yue Shang, Yi Li & Shu-zhen Chen 期刊:Acta Pharmacologica Sinica 影響因子:4.01 PMID:31235819
《Effects of nanoparticle size on the interaction between zinc oxide nanoparticles and bovine serum albumin》 作者:Liwei Wang, Tingting Liang, Jun Ma, Liqian Sun, Congcong Yang, Liqiang Meng et al. 期刊:Journal of Biomolecular Structure and Dynamics 影響因子:3.31 PMID:30938584
《NADPH Oxidase Isoforms Are Involved in Glucocorticoid-Induced Preosteoblast Apoptosis》 作者:Shu-Cai Bai,Qian Xu, Hui Li, Ya-Fei Qin, Li-Cheng Song, Chen-Guang Wang, Wen-Hao Cui, Zhi Zheng,De-Wen Yan,Zhi-Jun Li, Dong Li, Xin Wan,and Hua-Feng Zhang 期刊:Oxidative Medicine and Cellular Longevity 影響因子:4.868 PMID:31049140
《Activation of the p38/MAPK pathway regulates autophagy in response to the CYPOR-dependent oxidative stress induced by zearalenone in porcine intestinal epithelial cells》 作者:Tongtong Shen,Yufan Miao,Chenchen Ding,Wentao Fan,Shuhui Liu,Yanan Lv,Xiaona Gao,MartheDe Boevre,Liping Yan,Sheila Okoth,Sarah De Saeger,Suquan Song 期刊:Food and Chemical Toxicology 影響因子:3.775 PMID:31173817
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